Day 25 and 26: Result of my western blot: LC3B was identified successfully in all of the lanes: The brightness of the LC3B in each of the lanes: In lane 2: 2620, In lane 3: 4130, In lane 364, In lane 5: 1390, In lane 6: 3690. Plating the cells for this week: Immunofluorescence: The plates I split the cells that I had incubated in the flask in order to put them in the immunofluoresnce plates. Seeing as there were 24 wells in total, I wanted to plate enough cells for 28 wells to make up for pipetting error. 30, 000 cells per plate were required therefore 30, 000 x 28 = 840, 000. I found that after splitting the cells i had 997, 600 cells/ml. Thus, 840, 000 / 997, 600 = 842ul required. The amount of media required: 750ul x 28 = 21ml media. I had to sterilise coverslips to attach the cells to coverslips for subsequent confocal #microscopy. After sterilising the coverslips with 70% ethano...